Written by: The Midland Certified Reagent Company
Oligonucleotides have been researched for years and are becoming prevalent in today’s society.
The role of oligonucleotides in therapy has been increasing tenfold over the past ten years. This is due to the numerous clinical trials that have been occurring. While it may seem that there is still a good amount of time before an actual treatment is in circulation, there have been many beneficial developments that have come out of these trials.
More than a hundred types of oligonucleotides are candidates to be tested in a clinical setting, but only half of them are actually approved. Decades of research have brought the oligo synthesis process to this setting. Primarily, cancers are the leading candidate for oligos because of their ability to degenerate and kill off the malignant cells. Other disease forms that are being tested include: Duchenne’s muscular dystrophy, transthyretin amyloidosis, and corneal vascularisation.
Through both DNA and RNA synthesis, oligonucleotides have been altered in a laboratory setting to improve the rate at which malignant cells are being fought at. While cancer is known to spread at a rapid pace, scientists continue to develop new methods that reduce the number of limitations that prevent the oligo therapy from completely staving off infection.
RNA and DNA synthesis both play a role in antisense therapy. Through synthesis, the nucleic acid will bind to the messenger RNA and essentially deactivate the infected gene. Not only is this beneficial for research purposes, but there is a potential for this therapeutic treatment to become a major positive factor in today’s society. The various diseases that continue to be a major killer will only become worse until successful medical treatments are uncovered. This step is the first among the many on this road to finding a cure.
Written by: The Midland Certified Reagent Company
Oligos have come a long way in the research field.
In laboratories all over the world, oligonucleotides are being used for diagnostic and therapeutic purposes.
Prior to the dominance of oligos in the therapeutic world, they were primarily used as an aide to laboratory research. They were manufactured on a smaller scale because of their designated laboratory purpose. Now, they are in high demand due to their ability to assist in human diagnostics and drug resistance developments.
The increased production of both oligo and modified oligo nucleotides have led to more support in genetic methods such as bead-based assays, and isothermal amplification.
For studies of protein, researchers can figure out new, comprehensive strategies that will benefit both testing and researching phases. An advantage that this gives scientists is that they can carve a path ahead in developing new methods for the rest of the world to utilize.
Specific probes, like dual-labeled probes, can be used to assist in drug susceptibility and resistance issues in suffering countries like Africa. The hope is that their oligos will provide insight into creating a more resistant drug that will affect the human body in ways that will enhance and promote life.
As oligos continue to become a more mainstream and significant product in society, companies will heed the demands of laboratories and produce higher-grade oligos. This rapid movement is only the beginning of a positive trend towards revolutionary discoveries. New technologies will continue to be uncovered as genetic coding and oligo design continue to be relied upon when it comes time to make this leap.
Summary: Learn more about how beacon probes help scientists get closer to a cure for cancer.
A commonly used cytogenetic technique used to detect and localize tumor cells is known as fluorescence in situ hybridization (FISH). This involves a type of probe known as a molecular beacon probe, and is able to hone in on the location of tumor cells.
This method is non-radioactive and involves labeling molecular markers in order to more clearly detect hybridization. Medical techs seeking cancerous tumor cells look for specific complementary sequences of nucleic acids.
A typical synthetic beacon probe is long and thin, shaped like a hairpin. It is usually no more than 25 oligos long. Fifteen of the 25 nucleotides in a probe complement the desired target DNA or RNA, and the termini of the probe complement each other.
Biomedical researchers in the 1980s made the groundbreaking discovery that fluorescence microscopy can be used to detect a fluorescent probe binding to a specific chromosome.
When probes have a fluorescent label, the light emitted during the process of fluorescence resonance energy transfer (FRET) is visible in real time, ensuring that medical techs can very quickly locate tumor cells and track their metastasis.
While FISH is also occasionally used to identify species, its application in predictive and preventative medicine is very significant. Unusual temporal and spatial patterns of gene expression signify that cells and tissues are being invaded by a cancerous tumor, and FISH makes it easier for medical techs to find the tumor before it can metastasize.
Bio: The Midland Certified Reagent Company is a leader in the manufacture of polynucleotides for research purposes.
Summary: Using binary, we may be able to encode information on DNA.
Imagine a future where our information is stored inside of us. Sounds very science fiction like, but researchers at Harvard are making breakthroughs in that very field. Utilizing oligos, the team is using DNA as a binary storage device that they can write code to. The team can encode anything, using binary as a method of communication.
During oligo synthesis, synthetic strands are used like a printer. The “ink” in this case are the TG AC bases. If we take TG to mean “1” and AC to mean “0” we have the basis for binary communication. The sequence is encoded in binary. When the DNA strand is re-sequenced, the researchers are able to detect the binary code and store a whopping 700 terabytes of information for every gram of DNA.
The question is why anyone would think to store information inside of our DNA anyway.
Pros to DNA Storage
DNA storage has been on people’s minds for some time. You can store a surprisingly large amount of information in a relatively small space, and it’s durable too. DNA can survive for thousands of years in a box sitting in someone’s shed or in a warehouse.
The trouble has always been our ability, or lack thereof, to read DNA. The human genome consists of 3-billion base pairs, which we can only now begin to read for the first time. And it still takes hours of time.
This technology has a long way to go, but the future of DNA storage looks very bright.
Bio: The Midland Certified Reagent Company manufactures oligos, RNA polymers and synthetic materials used in medical research and experimentation. To order synthetic DNA, RNA or phosphorothioates, contact The Midland Certified Reagent Company.
Genetic diseases can be one of the most destructive to humans, and they can be difficult to fight. Making adjustments to the human genome is not a simple procedure, there are consequences for our actions. That’s why every medication used in human genetic immunization has to be thoroughly tested before it is deployed to market. Even testing must be cautious, but there are ways to test the effects on humans without resorting to human testing.
Utilizing Anti-Sense Therapy
Glioma affects the central nervous system, and is responsible for 80% of all malignant brain tumors. That’s a pretty significant chunk of cancer deaths, and one potential cause is genetics. Medical scientists have been exploring antisense drugs that target the human growth hormones, making necessary adjustments to the DNA, that may be a potential cure for this deadly form of cancer.
How it Works
Genes are a bit like computers in the sense that they follow specific instructions. Sometimes, just like computers, those instructions are bad and create a bug.
Antisense therapy relies on synthetic DNA or RNA that bonds with messenger RNA, or mRNA, to alter a particular gene and “debug” the problem. Although the process isn’t like staring at code at all. The genes are deactivated, or the mRNA could be told to bind with a splicing site. That would also alter its programming.
The virus known as AIDS is widely known, and well understood, but no clear cure exists. As we learn more about AIDS and its effects we have begun exploring solutions utilizing T-Cells. Unfortunately, this practice is also controversial in some countries and so a cure has not yet been realized. Still, early evidence proves promising.
Custom DNA sounds like a concept out of science fiction, but it’s become scientific reality and has been standard practice for many years. The usage of oligos has provided scientists with a greater understanding of microbiology, the life sciences, genetics and how our genes function. With this insight, we’ve been able to make great strides in curing disease and other problems humans face.
Human Testing is Expensive
Synthetic DNA is part of how scientists are able to test cures without harming humans. Human testing can be a dicey subject, and it’s not easy to come by. The prospect is also quite expensive. Synthetics like poly dC, poly dT, and poly dA enable researchers to simulate a reaction without the cost of performing it outside the laboratory.
Recreating an experiment in the laboratory also frees researches up to use colored dyes in order to observe reactions in greater detail. This is something that might otherwise be difficult or impossible in a human test subject, but oligonucleotides allow scientists to make observations by isolating and marking proteins.
It’s also important to understand that during these early phases, researchers are trying to study how DNA reacts to their supposed cure. This testing might have catastrophic effects if performed on a live subject. During these early phases, synthetics actually save lives.
Ideally, every cure tested on a human would have the desired result. However, synthetics are an important part of research during the early phases of trials.
Bio: The Midland Certified Reagent Company makes synthetic DNA for research purposes, including poly c, poly dC, poly dT and poly dA oligos. Contact Midland Certified Reagent Company to place an order for your institute.
Written By The Midland Certified Reagent Company
While just about everyone is familiar with DNA, most aren’t as read up on another important building block of life known as RNA. If you’re in this group, keep reading. We’ll discuss the important role RNA plays in your body and how modern geneticists are learning to use it for far greater means.
RNA stands for Ribonucleic Acid. Amongst other things, RNA is in charge of coding, expressing, decoding and regulating our genes. So genes contain a certain amount of instructions and our RNA is in charge of reading it and making sure our bodies follow those instructions.
Fortunately, nowadays, scientists have found out how they can artificially instigate RNA synthesis. This is important because the synthesis process is what gets RNA to work with DNA. Through this process, life is created. One of the major developments in this process is known as antisense oligonucleotides.
The truth is, as much as we know about RNA, we still don’t fully appreciate how much it can do. Or, rather, we don’t know how much we can do with it. The fact that we can now make artificial nucleotides to experiment on, though, means we’re about to get a much better idea in the near future.
Our understanding of RNA is integral to learning how it can be worked with in order to develop life-altering results.
The Midland Certified Reagent Company has been adding to the gains of modern science since 1978. Amongst other things, they have done tremendous work with both page purification and oligonucleotide synthesis.